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19–22 APRIL, 2017, BARCELONA, SPAIN
14:21–14:24
S1-11 (PP)
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CHARACTERIZATION OF RENAL PARENCHYMA
IMPAIRMENT IN PARTIAL UNILATERAL URETERAL
OBSTRUCTION IN MICE WITH INTRAVOXEL INCOHERENT
MOTION MR IMAGING
Maguelonne PONS
1
, Benjamin LEPORQ
2
, Liza ALI
3
, Marianne ALISON
4
,
Miguel ALBUQUERQUE
5
, Michel PEUCHMAUR
6
, Marie-Laurence POLI MÉROL
7
,
Ulrich BLANK
1
, Simon Auguste LAMBERT
2
and Alaa EL GHONEIMI
3
1) INSERM UMR 1149, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire d’excellence INFLAMEX, Paris,
FRANCE - 2) Univ Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREAT,
Villeurbanne, FRANCE - 3) Department of Pediatric Surgery and Urology, Hôpital Robert Debré, APHP, Université
Paris Diderot, S, Paris, FRANCE - 4) Department of Pediatric Radiology, Hôpital Robert Debré, APHP, Université
Paris Diderot, PRES Sorbon, Paris, FRANCE - 5) Pathology Deparment, Hôpital Beaujon, APHP, Clichy, FRANCE -
6) Department of Pathology, Hôpital Robert Debré, APHP, Université Paris Diderot, Sorbonne Paris Cité, Paris, FRANCE
- 7) Pediatric Surgery Unit, American Memorial Hospital, Université Reims Champagne Ardennes, Reims, FRANCE
PURPOSE
Obstructive nephropathy constitutes a major cause of progressive pediatric renal disease. We
propose to use intravoxel incoherent motion (IVIM) diffusion sequence to characterize kidney pa-
renchyma impairment on a clinically relevant mouse model of partial unilateral ureteral obstruction
(pUUO).
MATERIAL AND METHODS
The diffusion coefficient (Dslow), the perfusion coefficient (Dfast) and the perfusion fraction (f) were
extracted from IVIM data acquired on a 7T MRI. The imaging method was validated on 10 sham
wild type (WT) mice. Then 10 WT mice were subjected to UUOp at day 3 of life. At day 75, mice
underwent MRI examinations. Histological analysis was performed on both kidneys.
RESULTS
Diffusion parameters extracted from IVIM imaging were similar in both kidneys of sham WT mice.
Mean values of Dslow, Dfast and f were respectively 1.17±0.22mm2.s-1, 84.9±73.8mm2.s-1and
29.7±6.19% in the right kidney and 1.07±0.16mm2.s-1, 87.6±71.7mm2.s-1, and 30.6±7.0 % in the
left kidney. For pUUO mice a significant decrease of f (24.9±4.7 %) in the right operated kidney
compared to the sham right kidney was measured (p=0.04). Strong correlation between f and the
volume of the right kidney was observed (spearman coefficient=0.94, p=0.01) in severe pUUO
mice.
CONCLUSIONS
The IVIM sequence has been validated for the first time on mouse kidneys. According to the litera-
ture, our study suggests that a f reduction associated with a decrease volume parenchyma could
be related to a decrease of renal vascularization, appearing before fibrosis impairment. Perfusion
fraction is a good candidate as a MRI biomarker to follow quantitatively the early changes of kidney
pathophysiology in pUUO.