ESPU Congress 2017 - Abstract book

CONGRESS OF THE ESPU

14:18–14:21

S1-10 (PP)

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ESTABLISHMENT OF COLONIC DIALYSIS MODEL

IN UREMIC RATS BY RIGHT NEPHRECTOMY AND LEFT

PARTIAL NEPHRECTOMY

Nastaran SABETKISH

, Shabnam SABETKISH

and Abdol-Mohammad KAJBAFZADEH

1) Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells Therapy, Tehran Univ, Pediatric

Center of Excellence, Tehran, ISLAMIC REPUBLIC OF IRAN - 2) Tehran University of Medical Sciences, Pediatric

Urology and Regenerative Medicine Research Center, Pediatric Center of Excellence, Tehran, ISLAMIC REPUBLIC

OF IRAN - 3) Tehran University of Medical Sciences, Pediatric Urology and Regenerative Medicine Research Center,

Pediatric Center of Excellence, Tehran, ISLAMIC REPUBLIC OF IRAN

PURPOSE

To establish colonic dialysis (CD) model in uremic rats and to evaluate the effect of two different

peritoneal dialysis (PD) solutions.

MATERIAL AND METHODS

Twenty four male wistar rats were randomly divided into 3 groups. After applying right nephrectomy

and left partial nephrectomy, Malone antegrade continence enema (MACE) was created. Rats

of group I (N=8) received no postoperative intervention as control group. Seven days after the

procedure, blood sampling was performed and CD was started through the MACE stoma by a low-

osmolar PD solution in group II (N=8). Rats of group III (N=8), underwent the same procedure with

a high-osmolar PD solution. Mannitol and activated charcoal were also added to both PD solutions.

Weekly blood sampling was performed in order to evaluate the plasma creatinine and blood urea

nitrogen (BUN) level.

RESULTS

The respective mean±SD creatinine and BUN level was 1.5±0.04 and 49.5±5.6 mg/dl 7 days

after surgical procedure. While these values were in the steady low states after applying regular

CD in group III (0.6±0.02 and 24.1±2.8 mg/dl), they remained in higher levels in control group

(1.7±0.08 and 58.2±6.1 mg/dl, respectively) two weeks postoperatively. In the same time-point,

BUN and creatinine levels of rats in group II was 1±0.05 and 41.8±5.3 mg/dl. The mean±SD survival

period was 11±2, 20±3, and 33±2 days in the animals of group I, II, and III, respectively.

CONCLUSIONS

CD with an efficient PD solution through a MACE stoma may be a valuable option when conven-

tional methods are not available.