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19–22 APRIL, 2017, BARCELONA, SPAIN
14:15–14:18
S1-9 (PP)
EVAULATION THE EFFECT OF THE TESTIS FIXATION
IS PERFORMED THROUGH THE EPIDIDIYMO-TESTICULAR
JUNCTION ON ENFLAMATION, OXIDATIVE STRESS
AND SPERMATOGENESIS PARAMETERS IN RATS
Fatih ELBIR
1
and M.Selcuk SILAY
2
1) Midyat State Hospital, Urology, Mardin, TURKEY - 2) Medeniyet University, Pediatric Urology, Istanbul, TURKEY
PURPOSE
Undescended testis and testicular torsion operations are frequently being performed in pediatric
urology.We hypothesized that if the fixation suture is performed through the epididymotesticular
junction,we may cause less trauma to the testis.
MATERIAL AND METHODS
28 rats were assigned into 4 groups. First group was sham,the second group, fixation was per-
formed by passing the suture through the parenchyma, the third group through the ETJ and the
last group, testis was placed into the dartos pouch without fixation. Testicular tissues were exam-
ined histologically and biochemically. Inflammation, spermatogenesis, microlithiasis were criteria
of histopathological examination. The biochemical markers of inflammation and oxidative stress
parameters were evaulated.
RESULTS
The evaluation of spermatogenesis revealed that sham group was better than DPO and TETF
groups. When assesing inflammation parameters, in sham group, no inflammation was detected,
TPF group 1 testis had moderate inflammation, TETF group 1 testis had moderate inflammation
and 1 testis had severe inflammation, the DPO group 1 testis had moderate inflammation. The rate
of microlithiasis was significantly higher in TPF group when compared to the other groups. In the
sham group no microlithiasis was detected, the TPF group,at 6 testicles microlithiasis was detected
whereas in the TETF group, 2 testicles demonstrated microlithiasis, the DPO group only at 1 testis
microlithiazis was detected. The assessment of the inflammation markers revealed that IL-1B val-
ues for TPF and TETF groups were significantly higher than sham group. For IL-6,TPF group values
were significantly higher than sham and DPO groups.For TNF-α,TPF and DPO groups values were
significantly higher than sham group. In the testis tissues,TAS,TOS and OSI values were calculated.
TOS and TAS values found similar among all groups.However, for OSI, sham group value was
significantly lower than TPF and TETF groups.
CONCLUSIONS
Transparenchymal fixation leads histopathological and biochemical deterioration. Dartos pouch
was determinated the most reliable method. TETF method’s biochemical results were better than
TPF method so this shows that also reliable.