ESPU Congress 2017 - Abstract book

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12 28TH CONGRESS OF THE ESPU
14:12–14:15
S1-8 (PP)
IMMUNOHISTOCHEMICAL PROFILE OF NORMAL
PREPUBERTAL TESTES
Kolja KVIST1 , Erik CLASEN-LINDE2 , Dina CORTES3 and Jørgen THORUP1
1) Rigshospitalet, Pediatric Surgery, Copenhagen, DENMARK - 2) Rigshospitalet, Pathology, Copenhagen, DENMARK
- 3) Hvidovre Hospital, Pediatrics, Hvidovre, DENMARK
PURPOSE
We recently showed that normal infantile testes stain positive for PLAP, C-kit, D2-40 and Oct ¾ up
till 2 years of age, thereby stretching the time for completion of their maturation, a finding contrary
to previous studies.
This naturally begot the question as to when they become negative. This study aims to answer part
of that.
MATERIAL AND METHODS
Biopsies were taken from 11 boys, ages 11.5 to 16 years (mean 13,5, median 13), operated either
on suspicion of torsion of the testes or intermittent torsion.
The biopsies were processed for histology, cut into 4μm sections and mounted on coated slides.
One slide was processed for H-E, and the others incubated with primary antibody for PLAP, C-Kit,
D2-40 and Oct3/4.
RESULTS
Age
years
Diagnosis PLAP
dxt/sin
C-Kit
dxt/sin
D2-40
dxt/sin
Oct ¾
dxt/sin
11,5 intermittent pos/pos pos/pos neg/neg neg/neg
12,5 torsio dxt pos/ - pos/ - neg/ - neg/ -
14 torsio dxt pos/ - pos/ - neg/ - neg/ -
15 torsio dxt -/ pos - / pos - / neg - / neg
15 torsio dxt pos/ pos pos/ pos neg/ neg neg/ neg
13 torsio sin neg/ neg neg/ neg neg/ neg neg/ neg
16 torsio sin neg/ - neg/ - neg/ - neg/ -
13 intermittent pos/ neg pos/ neg neg/ neg neg/ neg
12 intermittent pos/ pos pos/ pos neg/ neg neg/ neg
15,5 intermittent pos/ pos pos/ pos neg/ neg neg/ neg
11,5 intermittent pos/ pos pos/ pos neg/ neg neg/ neg
CONCLUSIONS
To our knowledge, this is the first presentation of positive staining for PLAP and C-Kit in normal
pre-pubertal testes.
It attenuates that the maturation of germ cells is an ongoing process that apparently is not complete
before puberty.