ESPU Congress 2017 - Abstract book

19–22 APRIL, 2017, BARCELONA, SPAIN

15:41–15:44

S2-3 (PP)

IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES

IN BIOPSIES FROM NEUROGENIC BLADDERS USING

RNASEQ

Luise BORCH

, Søren HAGSTRØM

, Anne-Francoise SPINOIT

, Ulrik BAANDRUP

and Jane H CHRISTENSEN

1) Aarhus University Hospital, Pediatrics, Aarhus N, DENMARK - 2) Aalborg University Hospital, Pediatrics, Aalborg,

DENMARK - 3) Gent University Hospital, Urology, Gent, DENMARK - 4) North Denmark Reginal Hospital, Aalborg

University, Hjørring, Center for Clinical Reseach, Hjørring, DENMARK - 5) Aarhus University Hospital, Biomedicine,

Aarhus N, DENMARK

PURPOSE

The aim of this study was to analyze differentially expressed genes (DEGs) in bladder biopsies from

patients with a neurogenic bladder compared to controls.

MATERIAL AND METHODS

The study included two groups. Group 1 (controls): 13 adults with healthy bladders (6 males). Group

2: 11 adults with neurogenic bladders (5 males). One biopsy was retrieved from the bladder wall

of each subject. The biopsy was snap frozen in liquid nitrogen, followed by RNA extraction. RNA

sequencing data was obtained by next generation sequencing, and statistically significant DEGs

between the two groups were estimated. Enrichment of specific biological properties and biochemi-

cal pathways among the DEGs were identified.

RESULTS

Differential gene expression analysis identified 115 significant up-regulated DEGs, and 2 down-

regulated. SLURP1, keratins and UPK3A are among the top significantly regulated genes together

with their potential common regulator, KLF4.The DEGs are enriched in genes encoding secreted

protein and/or peptides andin genes with molecular functions in: 1) Serine-type endopeptidase

inhibitor activity, 2) Haptoglobin binding, and 3) RAGE receptor binding. The most significantly

enriched biological process among the DEGs is epidermis development. KEGG Pathway analysis

revealed 7 significantly enriched pathways (Q-value < 0.05). These pathways are predominantly

immune response pathways (including DEGs as IL6, IL8, CD20, CD21, CD22, CD36, CD42, and

AP1).

CONCLUSIONS

DEGs identified in biopsies from neurogenic bladder were predominantly enriched in immune

response pathways and the most regulated DEGs are likely involved in keratinocyte cell differ-

entiation, keratinization, signaling within non-neuronal cells through the α7 nicotinic acetylcholine

receptor as well as the barrier function of the urothelium.