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19–22 APRIL, 2017, BARCELONA, SPAIN
16:26–16:29
S2-11 (PP)
IMPACT OF REPEATED EXTRACORPOREAL SHOCK WAVE
LITHOTRIPSY ON PREPUBERTAL RAT KIDNEY
Jae Min CHUNG
1
, Won Yeol CHO
2
and Sang Don LEE
1
1) Pusan National University Yangsan Hospital, Urology, Yangsan-Si, REPUBLIC OF KOREA - 2) Dong-A University
Hospital, Urology, Pusan, REPUBLIC OF KOREA
PURPOSE
The study was aimed to investigate the effects of repeated extracorporeal shock wave lithotripsy
(ESWL) on the kidney of child and adult rats.
MATERIAL AND METHODS
Thirty rats were used; 15 were child rats (3 weeks of age) with an average body weight of 72.3 ±
3.3 g (range, 65-75 g) and 15 were adults with an average body weight of 265 ± 11.3 g (range,
250-280 g). The child and adult rats were separately and randomly allocated to three groups, each
consisting of five rats. Following anesthetization, the left kidney of each rat in each group received
3000 15 kV shock waves in one (group 1), two (group 2), or three (group 3) sessions. Sessions
were interspersed by 72 hours. The rats in each group were killed 72 hours after the last ESWL
session and both kidneys were harvested. The right kidney was used as the control. Renal injury
was examined with histological analysis, immunohistochemistry and Western blot to detecting the
expression of heat shock protein (HSP)-70, tumor necrosis factor (TNF)-α, intercellular adhesion
molecule (ICAM)-1, and monocyte chemoattractant protein (MCP)-1 as markers of renal damage.
RESULTS
HSP-70, ICAM-1, MCP-1, and TNF-α were similarly increased with increased ESWL sessions
in both age groups. Histological analysis revealed more serious fibrosis and inflammation in the
ESWL-treated kidneys in both groups compared to both age controls, with the damage increasing
with increasing numbers of sessions.
CONCLUSIONS
Kidney ESWL increased renal damage according to the number of sessions in both age groups of
rats. The effect of ESWL on renal injury was similar in both groups.
16:29–16:32
S2-12 (PP)
STEREOTACTIC TWO ACCESS MICRO PERCUTANEOUS
NEPHROLITHOTOMY: IN VIVO PIG MODEL EXPERIENCE
Onur TELLI
1
, Perviz HAJIYEV
1
, Uygar BAGCI
2
, Baris ESEN
2
, Tarkan SOYGUR
1
and Berk BURGU
1
1) Ankara University, Pediatric Urology, Ankara, TURKEY - 2) Ankara University, Urology, Ankara, TURKEY
PURPOSE
Micropercutaneous nephrolithotomy (micrPCNL) is one of the finest and most deliberate ap-
proaches for stone surgery. Despite having the advantage of using the finest single access possible,
thus causing less parenchymal damage, it has specific limitations like incapability of extracting
stone fragments and increased intrarenal pressure because of working in a closed system. If the