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RESULTS
Generally there were no significant differences in the composition of the microbiome between UB
and IS (p=0.528, Adonis). Both groups overlapped in principal component analyses. Shannon and
estimated Richness Indices were increased in UB samples, but did not reach statistical signifi-
cance (p=0.418; p=0.09). In detail, the microbial genera Pedobacter and Proprionibacterium were
significantly increased and Leucobacter was significantly decreased in native UB compared to IS
(p<0.05).
CONCLUSIONS
Following bladder augmentation, the native UB and IS (ileum or colon) host similar microbiota de-
spite the distinct differences of their mucosal barriers. Larger studies seem mandatory to investigate
whether the urinary microbiome contributes to the development of long-term histological and mucin
alterations in the neobladder mucosa following bladder augmentation.
16:35–16:38
S2-14 (PP)
ANALYSIS OF MICA AND ULBP-2 EXPRESSION
AND STABLISHMENT OF PATIENT DERIVED XENOGRAFTS
IN WILMS TUMOUR
Cristina RIÑON PASTOR
1
, Lucia FERNANDEZ CASANOVA
2
, Daniel AZORIN
CUADRILLERO
3
, Jaime VALENTIN QUIROGA
4
, Inmaculada DE PRADA VICENTE
3
and Antonio PEREZ MARTINEZ
5
1) Hospital Infantil Universitatio Niño Jesús, Pediatric Surgery / Pediatric Urology, Madrid, SPAIN - 2) Cnio (National
Centre of Oncological Investigatios), Clinical Investigation Unit., Madrid, SPAIN - 3) Hospital Infantil Niño Jesús,
Pathology, Madrid, SPAIN - 4) Hospital Universitario La Paz, Laboratorio De La Inmunidad Innata. Idipaz, Madrid,
SPAIN - 5) Hospital Universitario La Paz, Oncohaematology Unit, Madrid, SPAIN
PURPOSE
High risk renal tumours have a dismal prognosis. New approaches and treatments are needed.
Our aim is to identify in primary Wilms Tumour (WT) samples the expression of Major histocompat-
ibility complex class I-related chains A (MICA) and UL16 binding protein-2 (ULBP2), which are
recognized by the Natural Killer group 2 member D activating receptor (NKG2D), expressed on
Natural Killer (NK) cells, and to establish a WT xenograft model for the development of an NK cell
based immunotherapy.
MATERIAL AND METHODS
Between 2002 and 2014, we treated in our institution 42 patients with WT. We analyzed 28 primary
tumour samples, for MICA and ULBP2 expression. Intensity of cytoplasma staining and percentage
of positive cells were used to assess the levels of MICA and ULBP2. Matrigel coated tumour pieces
collected by surgical procedures were subcutaneously implanted on the dorsal region of NOD/scid
IL2rgnull (NSG) mice. SPSS was used for statistical analyses.
RESULTS
The percentage of MICA and ULBP2 positive cells was higher than 25% in 85.7% and 35.7%
respectively. Moderate-high intensity of stain was observed in 54.3% for MICA and 7.2% for ULBP2.
No correlation between MICA/ULBP2 expression and outcome was found. Only one sample from
a WT, not receiving preoperative chemotherapy, engrafted in NSG mice.