ESPU Congress 2017 - Abstract book

19–22 APRIL, 2017, BARCELONA, SPAIN

15:53–15:56

S2-7 (PP)

★

MACROPHAGE DEPLETION PRESERVES BLADDER

PHYSIOLOGY IN PARTIAL BLADDER OUTLET

OBSTRUCTION

Martin SIDLER

, Darius J BAGLI

and Karen J. AITKEN

1) The Hospital for Sick Children, Division of Pediatric Urology, Toronto, CANADA - 2) The Hospital for Sick

Children, Division of Pediatric Urology, Toronto, CANADA - 3) Research Institute, Hospital for Sick Children, Toronto,

Developmental and stem cell biology, Toronto, CANADA

PURPOSE

Macrophage play a pivotal role in tissue remodeling. We aimed to describe their practically unknown

role in obstruction-induced bladder remodeling.

MATERIAL AND METHODS

First, 12 female mice underwent partial bladder outlet obstruction (pBOO), half of them received

Clodronate liposome (CL) injections to deplete macrophages, 5 additional mice remained unoper-

ated; bladder-macrophages were quantitated using CyTOF after 2 weeks. Second, two groups

of 16 female mice underwent pBOO, half of each group received CL treatment. After two weeks,

micturition pattern was analysed before bladder harvest.

RESULTS

3.5% of cells from lysed bladders of unoperated mice were macrophages, while obstructed bladders

showed 17% macrophages, CL-treatment successfully reduced that ratio to 7% (pooled samples of

5 bladders each). CL-treatment in obstructed mice significantly increased mean voided volume by

almost 3-fold, similar to sham levels; CL-treatment also prevented obstruction-induced over-active

voiding pattern. In obstructed mice, we also observed a significantly increased bladder capacity

by CL-treatment by 50% compared to normal saline treated mice. qPCR and histologic workup is

pending.

CONCLUSIONS

We successfully established CL-treatment as method to reduce the number of bladder-macropha

ges. CL treatment preserved voiding efficiency and prevented obstruction-induced deterioration of

bladder physiology.

15:56–16:17

Discussion