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28
TH
CONGRESS OF THE ESPU
15:50–15:53
S2-6 (PP)
ADIPOSE STEM CELLS IN COMBINATION WITH PLATELET-
RICH PLASMA RESTORE MORPHORLOGY AND FUNCTION
OF URINARY SPHINCTER IN A NERVE-TRANSECTED RAT
MODEL OF STRESS URINARY INCONTINENCE
Gustavo VILLOLDO
1
, Romina ALBITE
1
, Jorge JAUNARENA
1
, Federico PEREYRA
BONNET
1
, Andrea SORDELLI
1
, Andrea SORDELLI
1
, Monica LORESI
1
,
Maximiliano DADAMO
1
, Walter GONZALEZ
1
, Marcelo IELPI
1
, Juan MOLDES
2
and Francisco DE BADIOLA
2
1) Hospital Italiano de Buenos Aires, Experimental Medicine, Ciudad De Buenos Aires, ARGENTINA - 2) Hospital
Italiano de Buenos Aires, Pediatric Urology, Ciudad De Buenos Aires, ARGENTINA
PURPOSE
Adipose derived stem cells (ADSC) are nonimmunogenic and have the ability to self-renew and
to differentiate into multiple cell types. They has been used to restore urinary sphincter function in
animal models. But there is not strong evidence regarding the role of the vehicle that must be used
in combination with ADSC to improve effectiveness at the injection site.
To assess whether adipose stem cell (ADSC) plus platelet rich plasma (PRP) could promote urethral
sphincter restoration in a stress urinary incontinence (SUI) rat model.
MATERIAL AND METHODS
Thirty five female inbred Wistar rats were used in our study. Animals were divided into seven
groups (five animals per group): continent (C), sham (S), PNT (D), PNT+PBS injection (P),
PNT+PBS+ADSC injection (PA), PNT+PRP injection (R) and PNT+PRP+ADSC injection (RA).
Twenty five females rats underwent bilateral pudendal nerve section (PNT) to induce SUI. ADSCs
were purified from fat tissue of a 4-week-old inbred male Wistar rat, labeled CM-Dil and injected
into the urinary sphincter in twelve o ́clock position with 70 microliltres of PBS or PRP. Four weeks
after injection, cystometry was undertaken in all animals and leak point pressure (LPP) measured
to assess urethral resistance function. All groups were sacrificed after cystometry, urethra sections
were submitted for histology, immunohistochemistry assessment.
RESULTS
LPP was increased significantly in R, RA and PA animals after implantation (P < 0.01), but was not
different from group C and S. Histological and immunohistochemical examination demonstrated
increased numbers of surviving ADSCs increased muscle/collagen ratio as well as increased nerve
fibers density surrounding ADSC at the injection sites in RA compared to PA animals (CM-Dil +).
CONCLUSIONS
PRP may potentially improve the action of transplanted ADSC to restore the histology and function
of the urethral sphincter in a SUI rat model.