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CONGRESS OF THE ESPU
stone is mobile in the collecting system the fixation prior to fragmentation can be challenging. We
aimed to define a new “Stereotactic Two Access Percutaneous Nephrolithotomy” technique which
is basically intersecting two-micro access sheets by using a 3-dimensional coordinating system
without additional radiation exposure and minimal parenchymal damage.
MATERIAL AND METHODS
14 female pigs with previously placed renal 10mm stones (plaster of Paris) were randomized in
two groups: classical micropcnl and stereotactic two access system. Catheter placed forperform
radiopaque injection and manometric recording during procedure. All access were performed by
one experienced surgeon for each group, after first access of PNL, in the second group the 3-di-
mensional coordinating system for the second access.
RESULTS
Stereotactic Two Access Percutaneous Nephrolithotomy was same focus without extra radiation.
This technique has the benefits of higher stone fragment extraction, less intrapelvic pressure during
the procedure, thus potentially causing less pyelonephritis.
CONCLUSIONS
In the second group for all (except one case) the second access was successfully achieved during
the first attempt by the device without any complication. The extracted stone fragment volume was
significantly higher in the stereotactic method, the mean pelvic-pressure was significantly lower and
the radiation exposure were similar between two groups.
16:32–16:35
S2-13 (PP)
THE URINARY BLADDER MICROBIOME FOLLOWING
AUGMENTATION IN CHILDREN
Zoltan Farkas KISPAL
1
, Peter VAJDA
2
, Daniel KARDOS
2
, Ingeborg KLYMIUK
3
,
Christine MOSSL-EICHINGER
4
, Georg SINGER
5
and Holger TILL
6
1) Medical University of Graz, Austria, Department of Paediatric and Adolescent Surgery,, Graz, AUSTRIA - 2) University
of Pecs, Surgical Division, Department of Paediatrics, Pecs, HUNGARY - 3) Medical University of Graz, Core Facility
Molecular Biology, Graz, AUSTRIA - 4) Medical University of Graz, Department of Internal Medicine, Joint Facilities,
Graz, AUSTRIA - 5) Medical University of Graz, Department of Paediatric and Adolescent Surgery, Graz, AUSTRIA -
6) Medical Universtiy of Graz, Department of Paediatric and Adolescent Surgery, Graz, AUSTRIA
INTRODUCTION
16s-rDNA-gene sequencing techniques provide novel insights into the microbiome of the urinary
bladder. The aim of the study was to individually compare the local microbiome of the native urinary
bladder (UB) with the different intestinal segments (IS) used for augmentation.
PATIENTS AND METHODS
Six patients with ileocystoplasty and 7 patients with colocystoplasty were included. Mean age at
the operation was 10 years. Histological samples were taken from the native UB and from the IS
used for augmentation during biannual postoperative follow-up (mean 8 (4-13) years). For microbial
analysis hypervariable regions V1-V2 were PCR amplified from total DNA isolates. Libraries were
analyzed using next-generation-sequencing on a MiSeq desktop sequencer (Illumina MiSeq). For
each patient microbial diversity and specific microbial genera of the UB were compared to the IS.