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28
TH
CONGRESS OF THE ESPU
S14: HYPOSPADIAS 2
Moderators: Enrique de la Peña (Spain), Alexander Springer (Austria)
ESPU Meeting on Friday 21, April 2017, 08:42–09:24
08:42–08:45
S14-1 (PP)
★
RANDOMIZED DOUBLE BLINDED CONTROLLED TRIAL
ON EPIDERMAL CHANGES IN PATIENTS SUBMITTED
TO SEX HORMONES TREATMENT PRIOR TO HIPOSPADIA
SURGERY – PRELIMINARY RESULTS
JoséMurillo NETTO
1
, Thaís CASALI
2
, Maria Christina CASTANON
3
, Matheus RODRIGUES
3
, Luana
PAIS
4
, Caroline SILVA
4
, Ubirajara BARROSO JR
5
, Kelly Christina PAIVA
6
and José BESSA JR.
7
1) Federal University of Juiz de Fora (UFJF) and Hospital e Maternidade Therezinha de Jesus of the Scho, Surgery/
Urology, Juiz De Fora, BRAZIL - 2) Federal University of Juiz de Fora (UFJF) and Hospital e Maternidade Therezinha
de Jesus of the Scho, Surgery / Plastic Surgery, Juiz De Fora, BRAZIL - 3) Federal University of Juiz de Fora (UFJF),
Morphology, Juiz De Fora, BRAZIL - 4) Federal University of Juiz de Fora (UFJF), Surgery/Urology, Juiz De Fora,
BRAZIL - 5) Federal University of Bahia (UFJA) and Bahiana School of Medicine, Surgery/Urology, Salvador, BRAZIL
- 6) Federal University of Juiz de Fora (UFJF) and Hospital e Maternidade Therezinha de Jesus of the Scho, Surgery/
Pediatric Surgery, Juiz De Fora, BRAZIL - 7) State University of Feira de Santana (UEFS), Surgery/Urology, Feira De
Santana, BRAZIL
PURPOSE
The rule of hormonal stimulation prior to hypospadias is still not clear. Studies have demonstrated
that estrogens improves recovery and healing of damaged tissue, diminishing fibrosis and providing
a better functional and aesthetic scar.
MATERIAL AND METHODS
Twenty-one children were randomized into three groups: CG: Neutral base ointment; TG:
Testosterone Propionate 1% ointment; EG: 0.01% estradiol ointment. Parents were oriented to
apply the ointment twice a day for 30 days prior to hypospadias surgery.
During surgery, fragments of foreskin were excised and fixed in 10% formalin. The skin fragments
were embedded in paraffin and sectioned for histology. Paraffin-embedded 4-µm sections were
stained with Hematoxilin Eosin (HE). For each sample of HE stained fragment the epidermis width
was measured in five different areas by image analysis using a Zeiss camera and PC image capture
system. The mean epidermis width (MEW) was calculated dividing the 5 measures by five.
RESULTS
There were 7 children in CG, 9 in TG and 5 in EG. There was no difference in the mean age and
hypospadias classification between the groups.
The MEW in CG was 18.74 ± 5.44 µm (13.68 - 29.69), in TG was 21.22 ± 8.29 µm (12.05 - 40.76),
and in EG was 32.85 ± 4.59 µm (26.16 - 36.79) (p=0.005). The epidermis had a greater width in EG
comparing to CG (p=0.005), and comparing to TG (p=0.016), and had the same width in CG and
TG (p=0.748).
CONCLUSIONS
These preliminary results demonstrate that the use of estrogen prior to hypospadias surgery
increases the width of foreskin epidermis. This improvement of skin quality may help preventing
complications after hypospadias surgery.