ESPU Congress 2018 - Abstract Book

22 29 th CONGRESS OF THE ESPU 14:30–14:33 S1-14 (PP) LONG TERM VOIDING DYSFUNCTION IN A MURINE MODEL OF BLADDER FUNCTION DISTURBANCE IN EARLY LIFE Nao IGUCHI  1 and Duncan WILCOX  2 1) University of Colorado AMC, Surgery, Aurora, USA - 2) Children's Hospital Colorado, Aurora, USA PURPOSE Disturbance of bladder function in early life contributes to lifelong bladder dysfunctions, however, the underlying mechanism remains to be elucidated. Voiding in neonatal mice depends on the perigenital-bladder reflex triggered by their mother licking the perineum until voluntary bladder control emerges. Therefore neonatal maternal separation (NMS) causes interference of normal bladder function in mice. This study aimed to examine the effects of early life voiding perturbation on long-term bladder function using NMS protocol in mice. MATERIAL AND METHODS Newborn mouse pups were divided into control and NMS groups. NMS pups were removed from their mother and housed individually (6 h/day) from postnatal day 2 to 14. Long-term effects of NMS on bladder function were assessed by void spot assays at 3–5 week-old, in vitro by detrusor contractility studies and urodynamic study (UDS) at 6 week-old. RESULTS NMS caused a significant decrease in muscarinic receptor-mediated detrusor contractility without affecting whole nerve-mediated contractility compared to control mice. NMS mice showed frequent small voids persistently even after control mice established voluntary void control. UDS showed detrusor overactivity in NMS mice and significantly decreased functional capacity compared with control group. CONCLUSIONS NMS caused an alteration of neural control of detrusor, a delayed establishment of regular voiding habits, and overactive bladder and decreased functional bladder capacity in late adolescent mice. Animal models with maternal separation protocol can provide a better understanding of mechanisms underlying bladder reflex maturation, which could be used in the future studies for the development of novel therapies to treat voiding dysfunction in children. 14:33–15:00 Discussion