ESPU Congress 2018 - Abstract Book

18 29 th CONGRESS OF THE ESPU MATERIAL AND METHODS Rat bladder smooth muscle (SMC) and SMC-like adipose-derived stem cells (pADSC) were co- cultured in a 1:1 ratio. Proteomic analysis of 1:1 cell mix secretome vs controls was performed to assess growth factor expression. Polyethylene glycol (PEG) or compressed collagen (CC) scaffolds were used alone or pre-seeded with SMCs and pADSC (1:1) prior to implantation into rat bladders after partial detrusorectomy. Bladders were harvested after four weeks, embedded in paraffin, sliced and analyzed for morphology, phenotype, vascularization and innervation. RESULTS Secretome analysis showed the presence of pro-angiogenic (VEGFA, ANG) and neurotropic fac- tors (NENF, GMFB, LIF, PTN, MANF) in the 1:1 cell mix, mostly enhanced compared to the SMC control. After detrusorectomy the defect was successfully covered by PEG and CC. After four weeks PEG alone was encapsulated and PEG+cells was mostly degraded. CC±cells did not cause inflam- mation, fully integrated into bladder with CC+cells exhibiting distributed vascularization, improved smooth muscle formation and neuronal ingrowth. CONCLUSIONS We have shown that the combination of CC with SMC-pADSC in 1:1 ratio is a promising scaffold for detrusor bioengineering in a rat detrusorectomy model and see this as a first step for whole bladder wall reconstruction. 14:18–14:21 S1-10 (PP) IN-VIVO REGENERATION OF BLADDER MUSCULAR WALL WITH DECELLULARIZED BLADDER MATRIX: AN EXPERIMENTAL STUDY Nastaran SABETKISH, Shabnam SABETKISH and Abdol-Mohammad KAJBAFZADEH Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem, Urology, Tehran, ISLAMIC REPUBLIC OF IRAN PURPOSE To determine histological aspects and biocompatibility of decellularized bladder graft for bladder augmentation using rat and rabbit models. MATERIAL AND METHODS Rat and rabbit bladders were decellularized and underwent different laboratory investigations to evaluate the efficacy of decellularization. Rats of control group (n=8) underwent sham surgery. After partial cystectomy in rats of the experimental group (n=8), the bladder was grafted with a patch of rat decellularized bladder. The same procedure was performed in 16 rabbits by the application of rabbit decellularized grafts. Biopsies were taken at 1, 3, and 9 months postoperatively for further histological investigations. RESULTS Total cell removal with preservation of extracellular matrix structure was confirmed in decellularized bladders. All bladders demonstrated a spherical shape without stone formation, or graft extrusion in none of the animals. Histological examination after 1 month of follow-up demonstrated few cells at the border of the graft in rats and rabbits. After 3 months of operation, the region of the graft was indistinguishable from natural bladder and continuity of transitional epithelium of natural blad- der on the decellularized grafted bladder tissue was confirmed. The organization of muscle layers