ESPU Congress 2018 - Abstract Book

16 29 th CONGRESS OF THE ESPU 13:48–13:51 S1-7 (PP) URETRAL REPLACEMENT USING TUBULARIZED COLLAGEN SCAFFOLD IMPLEMENTED WITH ADIPOSE DERIVED STROMAL CELLS Melodie JURICIC  1 , Kalitha PINNAGODA  2 , Marion BOURDENS  3 , Nicolas GAIDE  4 , Elisabeth JEUNESSE  4 , Matthias HANS LARSON  5 , Isabelle RAYMOND-LETRON  4 , Valerie PLANAT  3 and Olivier ABBO  1 1) Hôpital des Enfants - CHU Toulouse, Pediatric Surgery Department, Toulouse, FRANCE - 2) CHUV Lausanne -EPFL, Pediatric surgery -DMPC, Lausanne, SWITZERLAND - 3) Stromalab - UMR 1031 - Inserm - CNRS, Toulouse, FRANCE - 4) ENVT, Toulouse, FRANCE - 5) EPFL, Lausanne, SWITZERLAND PURPOSE Tissue engineering has emerged as a promising alternative approach in uretral reconstruction. Pinnagoda and al (Acta Biomat 2016) have successfully implanted an acellular double-layered collagen scaffolds as grafts for uretral replacement in a rabbit model. The value of cell-seeding scaffold to repair uretral defects has been underlined by several studies. Among the potential useful, Adipose derived stem cells (ADSCs) are well described, with differen- ciation, proangiogenic and immunomodulative properties. Therefore the aim of our study was firstly to seed ADSC into the previously described tubular col- lagen scaffold in order to analyze the in vitro features of the cells into the scaffold. Secondly we aimed to determine the role of the cells concerning the scaffold integration in a rabbit model of urethral defect. MATERIAL AND METHODS ADSCs were isolated from rabbit adipose tissue, then implemented during the polymerisation of the collagen tubularized scaffold. In vitro analysis concerned cellular morphology, viabily, proliferation and gene expression profile. In 10 New Zealand Rabbits, 2 cm long grafts were sutured to replace subtotal excision of uretra. They were prospectively randomised in 2 groups of 5 rabbits depending on the presence of seeded ADSC into the collagen scaffold. Histological and clinical results were compared after 10 days. RESULTS ADSCs within collagen scaffold retains characteristic morphology, are viable, proliferate, and have a gene expression profile comparable to ASCs growing in 2D culture. In vivo, clinical and histological evaluation 10 days after uretral reconstruction demonstrated integra- tion and early epithelialization in both groups. An increase inflammation appareance was observed in the « ASCs group » where the cells were still present and healthy in the implented group. CONCLUSIONS Tubiular Collagen scaffold seeded with ADSCs is feasible and may offer a useful alternative in the future for patients requiring tissular replacement. Further studies with longer. 13:51–14:12 Discussion