ESPU Congress 2018 - Abstract Book

15 11–14 APRIL, 2018, HELSINKI, FINLAND 13:45–13:48 S1-6 (PP) MICRORNA-132 IN BLADDER WOUND HEALING Xi LIU  1 , Clara Ibel CHAMORRO  1 , Dongqing LI  2 , Ning XU LANDÉN  2 , Behnaz Khalilzadeh BINICY  3 and Magdalena FOSSUM  4 1) Karolinska Institutet, Women's and Children's Health, Stockholm, SWEDEN - 2) Karolinska Institutet, Medicine, Stockholm, SWEDEN - 3) Örebro Universitet, Medicine, örebro, SWEDEN - 4) Karolinska Institutet/Karolinska Hospital, Women's and Children's Health/Patient Area for Children with urogenital malformation, Stockholm, SWEDEN PURPOSE In regenerative urogenital medicine, we hypothesize that a deeper understanding of normal bladder wound healing could further enlighten our understanding of essential factors related to regenerative medicine in healthy and diseased tissues. MicroRNAs (miRs) are a group of conservative small non-coding RNAs, crucial for post-transcrip- tional regulation in most biological events including wound healing. In skin, miR-132 has been reported as a key regulator for keratinocyte migration, proliferation and inflammation. The aim of this study was to analyse if miR-132 could be of importance to enhance urothelial cell migration and proliferation for wound closure. MATERIAL AND METHODS Human urothelial cells were isolated from bladder washings or biopsies. Cells were cultured until confluence before performing a standardized 2D scratch assay. The expression of miR-132 was analysed upon wounding after 6 h, 12 h, 24 h using real-time quantitative PCR by the – delta delta Ct with an internal control for U6 and a non-scratch control group (6 replicates per group). RESULTS The area of wound gap was significantly reduced within 24 h, miR-132 was up-regulated 1,8 times 6 h after wounding. At 12 h and 24 h after wounding, miR-132 was up regulated 1,4 times. Same results were found in primary urothelial cells isolated from bladder washes as from bladder biopsies. CONCLUSIONS Our results indicate that miR-132 expression was induced after wounding, and demonstrated a similar expression pattern as in normal skin wound healing. miR-132 could be an important factor for promoting urinary bladder wound healing, most probably by enhancing urothelial migration and suggests us to proceed with validations including functional studies.