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CONGRESS OF THE ESPU
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S1-14 (PP)
LONG TERM VOIDING DYSFUNCTION IN A MURINE MODEL
OF BLADDER FUNCTION DISTURBANCE IN EARLY LIFE
Nao IGUCHI
1
and Duncan WILCOX
2
1) University of Colorado AMC, Surgery, Aurora, USA - 2) Children's Hospital Colorado, Aurora, USA
PURPOSE
Disturbance of bladder function in early life contributes to lifelong bladder dysfunctions, however,
the underlying mechanism remains to be elucidated. Voiding in neonatal mice depends on the
perigenital-bladder reflex triggered by their mother licking the perineum until voluntary bladder
control emerges. Therefore neonatal maternal separation (NMS) causes interference of normal
bladder function in mice. This study aimed to examine the effects of early life voiding perturbation
on long-term bladder function using NMS protocol in mice.
MATERIAL AND METHODS
Newborn mouse pups were divided into control and NMS groups. NMS pups were removed from
their mother and housed individually (6 h/day) from postnatal day 2 to 14. Long-term effects of
NMS on bladder function were assessed by void spot assays at 3–5 week-old, in vitro by detrusor
contractility studies and urodynamic study (UDS) at 6 week-old.
RESULTS
NMS caused a significant decrease in muscarinic receptor-mediated detrusor contractility without
affecting whole nerve-mediated contractility compared to control mice. NMS mice showed frequent
small voids persistently even after control mice established voluntary void control. UDS showed
detrusor overactivity in NMS mice and significantly decreased functional capacity compared with
control group.
CONCLUSIONS
NMS caused an alteration of neural control of detrusor, a delayed establishment of regular voiding
habits, and overactive bladder and decreased functional bladder capacity in late adolescent mice.
Animal models with maternal separation protocol can provide a better understanding of mechanisms
underlying bladder reflex maturation, which could be used in the future studies for the development
of novel therapies to treat voiding dysfunction in children.
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Discussion