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th
CONGRESS OF THE ESPU
MATERIAL AND METHODS
Rat bladder smooth muscle (SMC) and SMC-like adipose-derived stem cells (pADSC) were co-
cultured in a 1:1 ratio. Proteomic analysis of 1:1 cell mix secretome vs controls was performed to
assess growth factor expression. Polyethylene glycol (PEG) or compressed collagen (CC) scaffolds
were used alone or pre-seeded with SMCs and pADSC (1:1) prior to implantation into rat bladders
after partial detrusorectomy. Bladders were harvested after four weeks, embedded in paraffin,
sliced and analyzed for morphology, phenotype, vascularization and innervation.
RESULTS
Secretome analysis showed the presence of pro-angiogenic (VEGFA, ANG) and neurotropic fac-
tors (NENF, GMFB, LIF, PTN, MANF) in the 1:1 cell mix, mostly enhanced compared to the SMC
control. After detrusorectomy the defect was successfully covered by PEG and CC. After four weeks
PEG alone was encapsulated and PEG+cells was mostly degraded. CC±cells did not cause inflam-
mation, fully integrated into bladder with CC+cells exhibiting distributed vascularization, improved
smooth muscle formation and neuronal ingrowth.
CONCLUSIONS
We have shown that the combination of CC with SMC-pADSC in 1:1 ratio is a promising scaffold for
detrusor bioengineering in a rat detrusorectomy model and see this as a first step for whole bladder
wall reconstruction.
14:18–14:21
S1-10 (PP)
IN-VIVO REGENERATION OF BLADDER MUSCULAR
WALL WITH DECELLULARIZED BLADDER MATRIX:
AN EXPERIMENTAL STUDY
Nastaran SABETKISH, Shabnam SABETKISH and Abdol-Mohammad KAJBAFZADEH
Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem, Urology,
Tehran, ISLAMIC REPUBLIC OF IRAN
PURPOSE
To determine histological aspects and biocompatibility of decellularized bladder graft for bladder
augmentation using rat and rabbit models.
MATERIAL AND METHODS
Rat and rabbit bladders were decellularized and underwent different laboratory investigations to
evaluate the efficacy of decellularization. Rats of control group (n=8) underwent sham surgery. After
partial cystectomy in rats of the experimental group (n=8), the bladder was grafted with a patch of
rat decellularized bladder. The same procedure was performed in 16 rabbits by the application of
rabbit decellularized grafts. Biopsies were taken at 1, 3, and 9 months postoperatively for further
histological investigations.
RESULTS
Total cell removal with preservation of extracellular matrix structure was confirmed in decellularized
bladders. All bladders demonstrated a spherical shape without stone formation, or graft extrusion
in none of the animals. Histological examination after 1 month of follow-up demonstrated few cells
at the border of the graft in rats and rabbits. After 3 months of operation, the region of the graft
was indistinguishable from natural bladder and continuity of transitional epithelium of natural blad-
der on the decellularized grafted bladder tissue was confirmed. The organization of muscle layers